The AccessQuick™ RT-PCR System is an easy and convenient master mix for one-tube RT-PCR. It increases the convenience of performing RT-PCR by combining the following components in a single tube: Tfl DNA Polymerase, dNTPs, magnesium sulfate and reaction buffer. The AMV RT enzyme is provided in a separate tube to allow important no-RT control reactions. The AccessQuick™ Master Mix is simply added to RNA templates in reaction vials, followed by the AMV RT, primers and water. The AccessQuick™ RT-PCR Master Mix is intended for routine RT-PCR applications that have been previously optimized and do not require extreme conditions.
Features – Benefits
- Maximum Convenience : Save yourself four pipetting steps. Simply combine the AccessQuick™ Master Mix, AMV RT, your gene-specific primers, your RNA template and water. Separate AMV RT allows important no-RT control reactions.
- Less Template : Amplify zeptomole (10–21mol) levels of RNA.
- No Buffer Additions Required : Set up reactions in a single tube, place in the thermal cycler and come back later for results—no additions between the reverse transcription and DNA amplification steps.
- Stability : System components are stable over many freeze-thaw cycles.
|AccessQuick™ RT-PCR System||20 reactions||Promega||A1701|
|AccessQuick™ RT-PCR System||100 reactions||Promega||A1702|
|AccessQuick™ RT-PCR System||500 reactions||Promega||A1703|