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GoTaq® Real-Time qPCR and RT-qPCR Systems for Dye-Based Detection

The GoTaq® qPCR Master Mix is optimized for fast and reproducible quantitative PCR assays using a double-stranded DNA-binding dye.

PROMOTION :

GoTaq® qPCR Master Mix, 200 reactions RP 4.000.000 (Valid until End of March 2017)

GoTaq® 1-Step RT-qPCR System, 200 reactions**(ED:Mar 2017) RP 5.000.000 (Valid until End of March 2017)

Product Description

The GoTaq® qPCR Master Mix is optimized for fast and reproducible quantitative PCR assays using a double-stranded DNA-binding dye. The master mix contains BRYT Green® dye, a novel fluorescent DNA-binding dye with minimal PCR inhibition for maximum PCR efficiency and greater fluorescence enhancement upon binding to double-stranded DNA (dsDNA) than SYBR® Green I.

The master mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs, BRYT Green® Dye and a proprietary reaction buffer. This master mix contains a low level of carboxy-X-rhodamine (CXR); a separate tube of CXR reference dye is included.

The GoTaq® 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System and GoTaq® qPCR Master Mix.

The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.

The GoTaq® 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® qPCR Master Mix in a single-step real-time amplification reaction.

The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities amounts of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions.

Features – Benefits

  • Achieve Superior Performance : Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range.
  • Rely on a Robust System : Resistant to a wide range of PCR inhibitors.
  • Use a Versatile Product : Compatible with both fast and standard cycling methods on most real-time PCR instruments.
  • Realize Enhanced Stability : Exceptional room-temperature setup is easy for automated and high-throughput detection.
Product Size Brand Catalog
GoTaq® qPCR Master Mix 5ml Promega A6001
GoTaq® qPCR Master Mix 25ml Promega A6002
GoTaq® 2-Step RT-qPCR System 5ml Promega A6010
GoTaq® 1-Step RT-qPCR System 5ml Promega A6020

 

Available Separately

Product Size Brand Catalog
CXR Reference Dye 100μl Promega C5411

 

Additional Information

Brand

Promega

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