The GoTaq® qPCR Master Mix is optimized for fast and reproducible quantitative PCR assays using a double-stranded DNA-binding dye. The master mix contains BRYT Green® dye, a novel fluorescent DNA-binding dye with minimal PCR inhibition for maximum PCR efficiency and greater fluorescence enhancement upon binding to double-stranded DNA (dsDNA) than SYBR® Green I.
The master mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs, BRYT Green® Dye and a proprietary reaction buffer. This master mix contains a low level of carboxy-X-rhodamine (CXR); a separate tube of CXR reference dye is included.
The GoTaq® 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System and GoTaq® qPCR Master Mix.
The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.
The GoTaq® 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® qPCR Master Mix in a single-step real-time amplification reaction.
The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities amounts of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions.
Features – Benefits
- Achieve Superior Performance : Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range.
- Rely on a Robust System : Resistant to a wide range of PCR inhibitors.
- Use a Versatile Product : Compatible with both fast and standard cycling methods on most real-time PCR instruments.
- Realize Enhanced Stability : Exceptional room-temperature setup is easy for automated and high-throughput detection.
|GoTaq® qPCR Master Mix||5ml||Promega||A6001|
|GoTaq® qPCR Master Mix||25ml||Promega||A6002|
|GoTaq® 2-Step RT-qPCR System||5ml||Promega||A6010|
|GoTaq® 1-Step RT-qPCR System||5ml||Promega||A6020|
|CXR Reference Dye||100μl||Promega||C5411|