GoTaq® Real-Time qPCR and RT-qPCR Systems for Probe-Based Detection

Description

The GoTaq® Probe qPCR Master Mix is optimized for fast and reproducible quantitative PCR assays in the hydrolysis probe detection format. The master mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl₂, dNTPs and a proprietary reaction buffer. This master mix does not contain a reference dye; a separate tube of CXR reference dye is included.

The GoTaq® Probe 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System and GoTaq® Probe qPCR Master Mix.

The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.

The GoTaq® Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® Probe qPCR Master Mix in a single-step real-time amplification reaction.

The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions. The GoTaq® Probe qPCR Master Mix includes dUTP. When dUTP is incorporated into the amplification products, the amplicons are susceptible to degradation by uracil-DNA glycosylase (UNG); allowing users to incorporate UNG into subsequent reactions for control of possible carryover contamination.

Features – Benefits

• Achieve Superior Performance : Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range.
• Rely on a Robust System : Resistant to a wide range of PCR inhibitors.
• Use a Versatile Product : Compatible with both fast and standard cycling methods on most real-time PCR instruments using TaqMan® and other probe assays such as molecular beacons.
• Realize Enhanced Stability : Exceptional room-temperature setup is easy for automated and high-throughput detection.