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GoTaq® Real-Time qPCR and RT-qPCR Systems for Probe-Based Detection

The master mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs and a proprietary reaction buffer. This master mix does not contain a reference dye; a separate tube of CXR reference dye is included.

PROMOTION :

GoTaq® Probe qPCR Master Mix 200 reaction 2ml A6101 (ED : April 2018) RP 1.200.000 (Valid until End of Augst 2017)

GoTaq® Probe 2-Step RT-qPCR System 200 reaction 2ml A6110 (ED : Januari 2018) RP 2.550.000 (Valid until End of Augst 2017)

GoTaq® Probe 1-Step RT-qPCR System 200 reaction 2ml A6120 (ED : Februari 2018) RP 2.950.000 (Valid until End of Augst 2017)

 

 

 

Product Description

The GoTaq® Probe qPCR Master Mix is optimized for fast and reproducible quantitative PCR assays in the hydrolysis probe detection format. The master mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs and a proprietary reaction buffer. This master mix does not contain a reference dye; a separate tube of CXR reference dye is included.

The GoTaq® Probe 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System and GoTaq® Probe qPCR Master Mix.

The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.

The GoTaq® Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® Probe qPCR Master Mix in a single-step real-time amplification reaction.

The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions. The GoTaq® Probe qPCR Master Mix includes dUTP. When dUTP is incorporated into the amplification products, the amplicons are susceptible to degradation by uracil-DNA glycosylase (UNG); allowing users to incorporate UNG into subsequent reactions for control of possible carryover contamination.

Features – Benefits

  • Achieve Superior Performance : Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range.
  • Rely on a Robust System : Resistant to a wide range of PCR inhibitors.
  • Use a Versatile Product : Compatible with both fast and standard cycling methods on most real-time PCR instruments using TaqMan® and other probe assays such as molecular beacons.
  • Realize Enhanced Stability : Exceptional room-temperature setup is easy for automated and high-throughput detection.
Product Size Brand Catalog
GoTaq® Probe qPCR Master Mix 2ml Promega A6101
GoTaq® Probe qPCR Master Mix 10ml Promega A6102
GoTaq® Probe 2-Step RT-qPCR System 2ml Promega A6110
GoTaq® Probe 1-Step RT-qPCR System 2ml Promega A6120

 

Additional Information

Brand

Promega

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