HaloTag® Mammalian Protein Purification System

The HaloTag® Mammalian Protein Purification System (Cat.# G6790) is an optimized kit for purification of HaloTag® fusion proteins from mammalian cell culture lysates.

Description

The HaloTag® Mammalian Protein Purification System (Cat.# G6790) is an optimized kit for purification of HaloTag® fusion proteins from mammalian cell culture lysates. HaloTag® fusion proteins form a highly specific and covalent bond with the HaloLink™ Resin. The covalent binding coupled with the low nonspecific binding of the HaloLink™ Resin provides superior purity and recovery of recombinant proteins from cultured mammalian cells, even at low expression levels. The HaloTag® Mammalian Protein Detection and Purification System (Cat.# G6795) also includes HaloTag® TMRDirect™ Ligand. The simple-to-use fluorescent detection of the HaloTag® fusion facilitates rapid optimization of expression and purification conditions.

Features – Benefits

  • Purify More Protein : HaloLink™ Resin covalently binds >7mg/ml of HaloTag® fusion protein (10X more capacity compared to FLAG®). Recovery is highly efficient, commonly >75%.
  • Higher Purity : Covalent capture allows extensive and/or stringent washes without loss of bound protein, resulting in very low (<0.1%) nonspecific binding and a highly pure protein.
  • Easily Scalable : Scale up and down, important for obtaining mg-plus quantities.
  • Optimized for Mammalian Protein Expression : The HaloTag® platform allows flexibility to move between purification, pull-downs and cellular imaging with a single construct.

Applications

  • Purification of HaloTag® fusion proteins from mammalian cell culture lysates.
Product Size Brand Catalog
HaloTag® Mammalian Protein Detection and Purification System 1 each Promega G6795
HaloTag® Mammalian Protein Purification System 1 each Promega G6790
HaloTag® Mammalian Protein Detection and Purification System Sample Pack 1 each Promega G6799

Available Separately

Product Size Brand Catalog
HaloTEV Protease 200μl Promega G6601
HaloTEV Protease 800μl Promega G6602
HaloTag® TMRDirect™ Ligand 30μl Promega G2991
Protease Inhibitor Cocktail, 50X 1ml Promega G6521

Additional information

Brand

Promega

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