M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant

Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT [H–]), Point Mutant, is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long RNA templates (>5kb).

Description

Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT [H–]), Point Mutant, is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long RNA templates (>5kb). The lack of RNase H activity is beneficial for this application, as RNase H can start to degrade templates when incubation times are long, as they may be when synthesizing long cDNAs. Although many researchers are successful in using M-MLV RT (H+) for analytical and some preparative cDNA applications, reverse transcriptases lacking RNase H activity provide another option to prepare long cDNAs and libraries containing a high percentage of full-length cDNA.

Features – Benefits

  • RNase H Minus : Provides optimal conditions to prepare full-length cDNA from long RNA templates.
  • Temperature Stability : Thermostability of this point mutant minimizes problems associated with secondary structure.
  • Increased Polymerase Activity : M-MLV RT (H–), Point Mutant, gives higher yields of cDNA compared with the deletion mutant (Cat.# M5301).
  • Provided with 5X Reaction Buffer : 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl2, 50mM DTT.
  • Broad Working Range : More tolerance to variations in enzyme and substrate concentrations means improved consistency in performance.

Applications

  • First-strand cDNA synthesis.
  • Primer extension.
Product Size Brand Catalog
M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant 2,500u Promega M3681
M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant 10,000u Promega M3682
M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant 50,000u Promega M3683

Additional information

Brand

Promega

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