ONE-Glo™ EX Luciferase Assay System

The ONE-Glo™ EX Luciferase Assay System provides both the high sensitivity and long-lived luminescence required to batch-process multiple plates in these assay formats. The ONE-Glo™ EX Assay retains many of the beneficial aspects of the ONE-Glo™ Assay, using 5´-fluoroluciferin substrate with an add-mix-read, or homogeneous, protocol.

Description

High- or ultrahigh-throughput quantitation of firefly luciferase expression in mammalian cells is commonly performed by measuring luminescence from 96-, 384- or 1,536-well plates. The ONE-Glo™ EX Luciferase Assay System provides both the high sensitivity and long-lived luminescence required to batch-process multiple plates in these assay formats. The ONE-Glo™ EX Assay retains many of the beneficial aspects of the ONE-Glo™ Assay, using 5´-fluoroluciferin substrate with an add-mix-read, or homogeneous, protocol. Extending the properties of ONE-Glo™ Reagent, ONE-Glo™ EX Reagent employs a new assay chemistry to increase the stability of both the luminescence signal and the reconstituted reagent. The approximately 2-hour signal half-life provides greater flexibility in assay design. A reconstituted reagent that can be stored at room temperature for longer periods means less variability in reagent performance during long experiments or screens and less sample waste. ONE-Glo™ EX Reagent is the firefly luciferase detection reagent used in the Nano-Glo® Dual-Luciferase® Reporter (NanoDLR™) Assay System, allowing the same reagent to be used for single- or dual-luciferase assays.

Features – Benefits

  • Experience Improved Handling : Increased stability of reconstituted reagent at room temperature or 4°C simplifies repeat use over long experiments and reduces waste; no odor-causing compounds in the reagent.
  • Achieve More Consistent Data : Bright and stable signal that can be measured for hours enables more constant luminescence readings; ideal for screening and batch processing.
  • Take Advantage of Workflow Flexibility : The homogenous firefly luciferase detection reagent can be used in both single- or dual-luciferase assays; compatible with any plate size up to 1,536-well format.
  • Notice Fewer Unwanted Effects from Sample Components : Reduced sensitivity to culture media, phenol red and luciferase inhibitors compared to other luciferase assays.

 

Product Size Brand Catalog
ONE-Glo™ EX Luciferase Assay System 10ml Promega E8110
ONE-Glo™ EX Luciferase Assay System 100ml Promega E8120
ONE-Glo™ EX Luciferase Assay System 10 x 10ml Promega E8130
ONE-Glo™ EX Luciferase Assay System 10 x 100ml Promega E8150

 

Additional information

Brand

Promega

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