pmirGLO Dual-Luciferase miRNA Target Expression Vector

The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3´ of the firefly luciferase gene (luc2). Firefly luciferase is the primary reporter gene; reduced firefly luciferase expression indicates the binding of endogenous or introduced miRNAs to the cloned miRNA target sequence.

Description

The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3´ of the firefly luciferase gene (luc2). Firefly luciferase is the primary reporter gene; reduced firefly luciferase expression indicates the binding of endogenous or introduced miRNAs to the cloned miRNA target sequence. This vector is based on Promega dual-luciferase technology, with luc2 used as the primary reporter to monitor mRNA regulation and Renilla luciferase (hRluc-neo) acting as a control reporter for normalization and selection.

Features – Benefits

  • Measure miRNA Function : Reporter activity correlates with miRNA activity.
  • Optimized Reporter Genes : luc2 gene provides highest expression.
  • Combination Renilla/Neomycin Marker : Normalize with Dual-Luciferase® Assay or for stable cell lines, all with one vector.
  • Biologically Relevant Results : The moderate-strength PGK promoter provides sensitive analysis not possible with strong promoters.

Applications

  • miRNA detection.
  • miRNA binding site analysis.
  • Transcript stability study.
  • 3´-UTR analysis.

 

Product Size Brand Catalog
pmirGLO Dual-Luciferase miRNA Target Expression Vector 20μg Promega E1330

 

Additional information

Brand

Promega

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