The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3´ of the firefly luciferase gene (luc2). Firefly luciferase is the primary reporter gene; reduced firefly luciferase expression indicates the binding of endogenous or introduced miRNAs to the cloned miRNA target sequence. This vector is based on Promega dual-luciferase technology, with luc2 used as the primary reporter to monitor mRNA regulation and Renilla luciferase (hRluc-neo) acting as a control reporter for normalization and selection.
Features – Benefits
- Measure miRNA Function : Reporter activity correlates with miRNA activity.
- Optimized Reporter Genes : luc2 gene provides highest expression.
- Combination Renilla/Neomycin Marker : Normalize with Dual-Luciferase® Assay or for stable cell lines, all with one vector.
- Biologically Relevant Results : The moderate-strength PGK promoter provides sensitive analysis not possible with strong promoters.
- miRNA detection.
- miRNA binding site analysis.
- Transcript stability study.
- 3´-UTR analysis.
|pmirGLO Dual-Luciferase miRNA Target Expression Vector||20μg||Promega||E1330|