QuantiFluor® dsDNA System

The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution.

Description

The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution. The quantitation of dsDNA is a very important step in many biological applications, particularly in standard molecular biology techniques. The dye shows minimal binding to single-stranded DNA (ssDNA) and RNA, allowing specific quantitation of dsDNA.

Features – Benefits

  • Experience Minimal Binding : Highly specific to dsDNA; minimal binding to ssDNA, RNA, protein and interfering compounds.
  • Increase Your Sensitivity : Significantly increased sensitivity compared to absorbance at 260nm (NanoDrop® spectrophotometer) for low-concentration samples. Performs better or equal to PicoGreen® dye and can detect as little as 50pg/ml.
  • Set Up Quickly and Easily : System includes all required reagents to quickly set up and quantitate dsDNA.
  • Use with Promega Instruments : Pre-optimized on both the Quantus™ Fluorometer and GloMax®-Multi+ Instrument.
  • Use for Next-Gen Sequencing : Successfully used in several Next-Gen Sequencing systems, including Illumina (HiSeq/miSeq), Roche (454) and LifeTech (ion Torrent and ion Proton) systems.

Applications

  • Quantification of double-stranded DNA using fluorescent methods.
  • Quantification of dsDNA prior to next-gen sequencing (see citations).
Product Size Brand Catalog
QuantiFluor® dsDNA System 1ml Promega E2670
QuantiFluor® dsDNA Sample Ki 1 each Promega E2671

 

Available Separately

Product Size Brand Catalog
0.5ml PCR Tubes 50 pack Promega E4941
0.5ml PCR Tubes 200 pack Promega E4941

Additional information

Brand

Promega

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