Description
The GoTaq® qPCR Master Mix consists of 2X Master Mixes containing of BRYT Green® Dye which is a fluorescent DNA Binding dye with minimal PCR inhibition, providing maximum amplification efficiency and greater fluorescence enhancement than SYBR® Green I. Rapid hot-start activation and processive enzymes make the GoTaq® Systems compatible with most real time PCR instruments using standard or fast cycling.
The GoTaq® qPCR Master Mix is a ready-to-use 2X master mix for real-time quantitative PCR. Combining GoTaq® Hot Start Polymerase, optimized buffer and the BRYT Green® Dye, the GoTaq® qPCR Master Mix provides robust real-time PCR with earlier quantification cycle values and broad-range detection. These qualities result in increased reliability, reproducibility and sensitivity for quantitative, real-time PCR.
Designed for use with any instrument capable of reading SYBR® Green I or FAM™ Dye, the GoTaq® qPCR Master Mix offers:
- Sensitive detection for earlier quantitation of low- and high-copy targets.
- Room-temperature setup, making it suitable for automation and high-throughput detection.
- Compatibility with both fast and standard qPCR cycling methods.
Features – Benefits
- Ready-to-use mix of optimized components for dye-based qPCR or RT-qPCR
- Compatible with both fast and standard cycling methods
- Resistant to a wide range of PCR inhibitors
| Product | Size | 10µl Reactions | 20µl Reactions | Brand | Catalog |
| GoTaq® qPCR Master Mix | 5 ml | 1000 reactions | 500 reactions | Promega | A6001 |
| GoTaq® qPCR Master Mix | 25 ml | 5000 reactions | 2500 reactions | Promega | A6002 |
Specifications
| Item | Size | Catalog |
| GoTaq® qPCR Master Mix, 2X | 5 × 1 ml | A600A |
| CXR Reference Dye | 1 × 100μl | C541A |
| Nuclease-Free Water | 2 × 13 ml | P119E |
