Description
The GoTaq® Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® Probe qPCR Master Mix in a single-step real-time amplification reaction. The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions. The GoTaq® Probe qPCR Master Mix includes dUTP. When dUTP is incorporated into the amplification products, the amplicons are susceptible to degradation by uracil-DNA glycosylase (UNG); allowing users to incorporate UNG into subsequent reactions for control of possible carryover contamination.
The GoTaq® Probe 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System and GoTaq® Probe qPCR Master Mix. The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.
Features – Benefits
- Achieve Superior Performance: Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range.
- Rely on a Robust System: Resistant to a wide range of PCR inhibitors.
- Use a Versatile Product: Compatible with both fast and standard cycling methods on most real-time PCR instruments using TaqMan® and other probe assays such as molecular beacons.
- Realize Enhanced Stability: Exceptional room-temperature setup is easy for automated and high-throughput detection.
| Product | Size | 10µl Reactions | 20µl Reactions | Brand | Catalog |
|---|---|---|---|---|---|
| GoTaq® Probe 1-Step RT-qPCR System | 2 ml | 400 reactions | 200 reactions | Promega | A6120 |
| 12.5 ml | 2,500 reactions | 1,250 reactions | Promega | A6121 | |
| GoTaq® Probe 2-Step RT-qPCR System | 2 ml | 50 × 20µl RT reactions and 400 × 10µl qPCR reactions | 50 × 20µl RT reactions and 200 × 20µl qPCR reactions | Promega | A6110 |
Specifications GoTaq® Probe 1-Step RT-qPCR System
| Product | GoTaq® Probe 1-Step RT-qPCR System | GoTaq® Probe 2-Step RT-qPCR System |
||
| Size | Catalog | Size | Catalog | |
| GoTaq® Probe Master Mix with dUTP | 1 × 12.5 ml | A612B | 2 × 1ml | A612A |
| CXR Reference Dye (30μM) | 1 × 500μl | C541C | 1 × 200μl | C541B |
| GoScript™ RT Mix for 1-Step RT-qPCR | 1 × 500μl | M700C | 1 × 225μl (50X) | M700A |
| Nuclease-Free Water | 1 × 13 ml | P119E | 2 × 1,250μl | P119A |
Specifications GoTaq® Probe 2-Step RT-qPCR System
| Item | Size | Catalog |
| MgCl2 (25 mM) | 1 × 750μl | A351B |
| GoScript™ 5X Reaction Buffer | 1 × 300μl | A500B |
| GoScript™ Reverse Transcriptase | 1 × 50μl | A501B |
| GoTaq® Probe qPCR Master Mix, 2X | 2 × 1ml | A610A |
| Oligo(dT)15 Primer (500μg/ml) | 1 × 50μg | C110B |
| PCR Nucleotide Mix (10 mM) | 1 × 200μl | C114G |
| Random Primers | 1 × 50μg | C118B |
| CXR Reference Dye | 1 × 100μl | C541A |
| Recombinant RNasin® Ribonuclease Inhibitor (40u/μl) | 1 × 2,500u | N251A |
| Nuclease-Free Water | 3 × 1,250μl | P119A |
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