PowerPlex® Fusion Systems

Description

Designed to meet CODIS and European standards, the PowerPlex® Fusion Systems enable laboratories to:

• Achieve the most inter-database compatibility and highest discrimination of any autosomal STR kit.
• Improve laboratory efficiencies with rapid-cycling and direct-amplification protocols.
• Obtain a higher success rate with difficult casework samples due to robustness and sensitivity and the inclusion of three Y-STR loci for improved mixture interpretation.
• Simplify validation and QC efforts by using one kit for both casework and databasing sections.

The PowerPlex® Fusion Systems offer the highest discrimination from a single reaction and deliver more information in demanding forensic, paternity and relationship-testing cases.

As DNA databases continue to grow and international cooperation increases, the need for a common set of markers is required to facilitate data sharing and to reduce adventitious matches. The PowerPlex® Fusion and Fusion 6C Systems enable increased discriminatory power and data-sharing possibilities by common and informative loci used throughout the world, such as SE33, Penta D and Penta E.

A probability of identity value (PI) is defined as the probability that two individuals selected at random will have an identical genotype at the tested locus. It is calculated by adding the square of the genotype frequencies. The figure indicates the Probability of Identity values for a variety of currently available STR systems and standards. A significant increase in discriminatory power is seen with the PowerPlex® Fusion Systems over other well established STR systems. Data were kindly provided by John Butler (NIST) using data collected by Carolyn Hill and analysis developed by Dave Duewer.

Maximum Sensitivity

The PowerPlex® Fusion System reliability produces complete STR profiles from as little as 100pg of human DNA.

Superior Inhibitor Tolerance

With a robust buffer system designed to tolerate the most challenging inhibitors, the PowerPlex® Fusion System minimizes the need to re-amplify samples generally thought to be too challenging to run, saving your lab countless hours of repeat analysis.

Direct-Amplification Compatibility for Most Sample Types

Building on the proven flexibility of our PowerPlex® 21 System, the PowerPlex® Fusion System allows direct amplification from FTA® card punches as well as pretreated nonFTA cards and commonly used swabs to streamline workflow and expand sample throughput.