ReliaPrep™ FFPE gDNA Miniprep System

Description

The ReliaPrep™ FFPE gDNA Miniprep System provides a complete, all-inclusive method for purifying quality genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Extraction of DNA from FFPE tissues is challenging because the formalin fixation process results in cross-linking between proteins and DNA, as well as between different strands of DNA. This nonoptimal preservation of genomic DNA in FFPE complicates its use in many standard downstream analysis applications.

The ReliaPrep™ FFPE gDNA Miniprep chemistry is optimized to partially reverse this cross-linking without using hazardous solvents or overnight digestion. Genomic DNA can be isolated from FFPE tissue in approximately two and one-half hours with minimal hands-on time.

Features – Benefits

• Isolate Quality, Intact gDNA: Optimized lysis and binding conditions reverse modifications introduced by the fixation process, resulting in intact, amplifiable gDNA.
• Safely Deparaffinize Your Sample: Deparaffinization step occurs without harsh organic solvents.
• Save Time: Purify gDNA from FFPE tissue in less than two and one-half hours with minimal hands-on time. No overnight digestion required.
• Easy to Use: Minimal preparation time; simply add ethanol and go!

Applications

• Purification of genomic DNA from formalin-fixed, paraffin-embedded tissue (FFPE).

For more specifications, click here