ReliaPrep™ FFPE Total RNA Miniprep System

Description

Formalin fixation and paraffin embedding (FFPE) is a common method for archiving tissue specimens. The ability to extract RNA from these samples provides the potential for correlating disease state and tissue morphology with gene expression. Historically, extraction of RNA from FFPE tissues was a challenge because the formalin fixation process cross-links proteins and nucleic acids and introduces modifications to RNA by addition of monomethyl groups to nucleotides (1,2). These modifications can inhibit subsequent analysis of RNA isolated from FFPE tissues.

The ReliaPrep™ FFPE Total RNA Miniprep System optimizes lysis conditions to reverse these RNA modifications without the need for overnight digestion. This system also uses a deparaffinization method that does not rely on xylene or other organic solvents. By eliminating the washing steps involved in xylene deparaffinization, this method can help retain small RNA fragments.

Features – Benefits

• Easy to Use: Minimal preparation time.
• Safe: Deparaffinization step occurs without harsh organic solvents.
• Isolate Quality, Intact Total RNA: Fine-tuned protocol results in high-quality, intact, amplifiable total RNA.

Applications

• Purification of total RNA from formalin-fixed, paraffin-embedded tissue (FFPE).

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