Description
The ReliaPrep™ Large Volume HT gDNA Isolation System isolates genomic DNA (gDNA) from 1–10ml of blood in a scalable format. The chemistry eliminates tedious centrifugation steps as well as the use of hazardous chemicals, which are inherent in precipitation-based chemistries. Each reagent kit provides enough reagents to process up to 96 × 10ml whole blood samples. The system has been automated on robotic liquid-handling workstations, allowing walkaway purification of genomic DNA from 1–10ml of whole blood, regardless of sample storage or shipping conditions. For low-throughput isolation of gDNA from up to 32 samples at one time, the HSM 2.0 can be used in a manual mode, where the user performs the pipetting functions. The HSM has software that controls the instrument and directs the user through the purification protocol.
Features – Benefits
- Decrease Hands-On Time: Automation reduces operator time spent on instrument setup and takedown by allowing walkaway operation for large numbers of samples at one time.
- Remove Protocol Bottlenecks: Heater Shaker Magnet eliminates the need to move samples on the robot deck, reducing instrument failures; precipitation-free chemistry dramatically reduces purification failures.
- Achieve Peace of Mind: Automated liquid level sensing with operator notification allows recovery of samples in case of error.
- Isolate Pure DNA from All Samples: Purification chemistry is equally effective at recovering DNA from pristine as well as challenged (hemolysed or frozen) samples.
- Save a Day or Two of Processing: Samples are eluted in buffer, ready for use in downstream assays or archiving, eliminating resuspension of pelleted DNA, which can take 24–48 hours.
- Reduce Waste: Chemistry is automatically scaled for each sample and plastic use is conserved, reducing liquid and solid waste during sample runs.
Applications
- DNA isolation from 1–10ml of blood.
- Automated, walkaway sample processing.
| Product | Size | Brand | Catalog |
| Maxwell® RSC Plant RNA Kit | 48 preps | Promega | AS1440 |
Specifications
| Item | Size | Catalog |
| HSM 2.0 Instrument | 1 each | A2715 |
| Alkaline Protease (APA) | 1 × 130ml | A1721 |
| Cell Lysis Buffer (CLD) | 1 × 1400ml | A1731 |
| Binding Buffer (BBA) | 1 × 1600ml | A1741 |
| ReliaPrep™ Resin | 1 × 115ml | A1752 |
| Prepared Wash Buffer (WBC) | 1 × 3500ml | A2681 |
| Proteinase K (PK) Solution | 1 × 23ml | A5051 |
| Nuclease-Free Water | 4 × 150 ml | P1197 |
Available Separately
| Product | Size | Catalog |
| RNase A Solution | 5 ml | A7974 |
| 20X TE Buffer (pH 7.5) | 25 ml | A2651 |
| Tissue Lysis Buffer (TLA) | 500 ml | A5091 |
| Nuclease-Free Water | 1000 ml | P1199 |
| Integrated Reagent Caps | 4/pk | A2701 |
| HSM 2.0 Instrument Cover | 1 each | A2712 |
| HSM 2.0 Tube Rack | 1 each | A2713 |
| HSM 2.0 Tube Rack Stand | 1 each | A2714 |
| HSM 2.0 Instrument 1-Year Service Agreement | 1 each | SA1330 |
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