Wizard® SV Gel and PCR Clean-Up System

The Wizard® SV Gel and PCR Clean-Up System is designed to extract and purify DNA fragments of 100bp to 10kb from standard or low-melting agarose gels or to purify products directly from PCR and other common reactions such as restriction digests.

Description

The Wizard® SV Gel and PCR Clean-Up System is designed to extract and purify DNA fragments of 100bp to 10kb from standard or low-melting agarose gels or to purify products directly from PCR and other common reactions such as restriction digests. Up to 95% recovery is achieved depending upon the DNA fragment size. PCR products are commonly purified to remove excess nucleotides and primers. This membrane-based system, which can bind up to 40μg of DNA, allows recovery of isolated DNA fragments or PCR products in as little as 15 minutes, depending on the number of samples processed. The purified DNA can be used for automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion or in vitro transcription/translation without further manipulation.

Features – Benefits

  • Improved Productivity: Purify DNA fragments or PCR products in as little as 15 minutes.
  • Enhanced Cloning Results: Up to 95% recovery eluted in as little as 15μl.
  • Confidence in Results: Purified DNA routinely achieves 700 bases with >98% accuracy in automated fluorescent sequencing.
  • Applications Tested: DNA is suitable for automated fluorescent sequencing, cloning, labeling, restriction enzyme digestion or in vitro transcription/translation without further manipulation.
  • One System to Do It All: One system can replace up to four kits from other suppliers.
Product Size Brand Catalog
Wizard® SV Gel and PCR Clean-Up System 50 preps Promega A9281
Wizard® SV Gel and PCR Clean-Up System 250 preps Promega A9282
Wizard® SV Gel and PCR Clean-Up System 1000 preps Promega A9285
Wizard® SV Gel and PCR Clean-Up System and x-tracta™ Gel Extractor Bundle 50 preps/25 extractors Promega A9283
Wizard® SV Gel and PCR Clean-Up System and x-tracta™ Gel Extractor Bundle 250 preps/100 extractors Promega A9284
Wizard® SV Gel and PCR Clean-Up Start-Up Kit, 110V Electrical (500 preps, manifold and free vacuum pump) 1 each Promega A6750
Wizard® SV Gel and PCR Clean-Up Start-Up Kit, 220V Electrical (500 preps, manifold and free vacuum pump) 1 each Promega A6752
Wizard® SV Gel and PCR Clean-Up Start-Up Kit, 100V Electrical (500 preps, manifold and free vacuum pump) 1 each Promega A6754

Specifications

Item Size Catalog
Wizard® SV Minicolumns 1 × 50/pk A129A
Collection Tubes 1 × 50/pk A130A
Membrane Wash Solution 1 × 15ml A929B
Membrane Binding Solution 1 × 20ml A930B
Nuclease-Free Water 3 × 1,250μl P119A

 

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Additional information

Brand

Promega

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