Description
Bacterial Strain JM109(DE3), derived from JM109, contains a chromosomal copy of the gene for T7 RNA polymerase. JM109(DE3) is used for the high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, provided that the cloned sequence contains a ribosomal binding site. Please note: JM109(DE3) cannot be used for blue/white selection.
Genotype: endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, λ–, Δ(lac-proAB), [F´, traD36, proAB, lacIqZΔM15], lDE3.
Features – Benefits
- Contains an IPTG-inducible gene for T7 RNA polymerase
- Requires a ribosomal binding site with cloned sequence
- Lacks blue/white cloning selection
| Product | Size | Brand | Catalog |
| Bacterial Strain JM109(DE3) | 500ul | Promega | P9801 |
Specifications
| Product | Size | Catalog |
| Bacterial Strain JM109(DE3) | 1 × 500μl | P980A |
