Gels & Buffers

Description

COLORED LAEMMLI STACKING GEL SOLUTION

Overview

• Premixed acrylamide, stacking gel buffer, pink dye
• Room temperature stable
• Forms colored stacking gel for easy loading
• Compatible with downstream applications

Detailed Description

AMRESCO’s Colored Laemmli Stacking Gel Solution is a convenient mixture of acrylamide, stacking gel buffer and inert pink dye, which requires only the addition of ammonium persulfate and TEMED to form a stacking gel. The pink dye enhances the visibility of the sample wells to simplify protein loading, without interfering with the performance of the gel. The gel may be used with all standard downstream applications, such as gel staining and Western blotting.

STACKING GEL BUFFER, 4X

The 4X concentrated buffer (0.5 M Tris pH 6.8 and 0.4% SDS) for preparation of stacking gels for Laemmli SDS-Page. Dilution to a 1X solution results in final 1X concentrations of 0.125M Tris and 0.1% SDS

SEPARATING GEL BUFFER, 4X

4X concentrated buffer solution (1.5 M Tris, pH 8.8 and 0.4% SDS). Used for preparation of standard acrylamide: bis-acrylamide gels as well as preparation of gels using AMRESCO’s PAGE-PLUS™ acrylamide solution.

PROTEIN EZ-VISION®, 4X

Overview

• Immediate visualization
• Sensitivity down to 100 ng protein
• Compatible with downstream Western blotting

Detailed Description

Protein EZ-Vision® is non-hazardous, flourescent reagent that produces instant visualization of protein bands upon UV illumination of SDS-PAGE gels. Supplied in a 4X loading buffer, Protein EZ-Vision® comigrates with the protein-SDS complex during electrophoresis. Post-run staining and destaining is completely eliminated and results can be visualized immediately after the run by placing the gel on a standard UV transilluminator. Available in 2 x 1 ml tubes (KIT) or in a 0.2 ml sample size.