GoTaq® G2 Hot Start Polymerase and Master Mixes

Description

GoTaq® G2 Polymerase is the second generation of GoTaq® products. It comes in a variety of formats designed to provide maximum flexibility, control and convenience.

For superior convenience and improved yield, sensitivity and specificity, choose GoTaq® G2 Hot Start Polymerase, which is bound to a proprietary antibody that blocks activity. Activity is restored during initial denaturation, allowing hot-start PCR. Available as a master mix or standalone enzyme.

GoTaq® G2 Hot Start Polymerase is supplied with 5X Green GoTaq® Flexi Buffer, 5X Colorless GoTaq® Flexi Buffer and 25mM MgCl₂. The high-performance GoTaq® G2 DNA Polymerase is bound to a proprietary antibody that blocks polymerase activity. Polymerase activity is restored during the initial denaturation step, when amplification reactions are heated at 94–95°C for two minutes, allowing hot-start PCR in which polymerase activity is inhibited at temperatures below 70°C for convenient, room-temperature reaction setup. Hot-start PCR is advantageous for some amplification targets because it may eliminate or minimize primer-dimer and nonspecific products. In some cases, hot-start PCR may improve yields. GoTaq® G2 Hot Start Polymerase exhibits 5´→3´ exonuclease activity.

The GoTaq® G2 Hot Start Master Mixes are ready-to-use mixes containing all necessary components (GoTaq® G2 Hot Start Polymerase, buffer, dNTPs and optimized magnesium)—you only need to add primer and template and go!

The GoTaq® G2 Hot Start Green Master Mix also contains a gel loading dye to facilitate downstream gel analysis. The GoTaq® G2 Hot Start Colorless Master Mix contains no gel loading dye for use when downstream applications require fluorescence or absorbance readings without purification.

Features – Benefits

Robust reliable PCR for all your amplification needs.

• Simplify reaction setup and save time with a ready-to-use master mix.
• Prepare your reaction at room temperature, not on ice.
• Eliminate nonspecific amplification with hot-start enzyme.
• Use at no risk—backed by the Promega PCR Satisfaction Guarantee.