Description
The GTPase-Glo™ Assay measures GTPase activity by detecting the amount of GTP remaining after GTP hydrolysis in a GTPase reaction. After the GTPase reaction, addition of the GTPase-Glo™ Reagent converts any remaining GTP into ATP, which is converted to a luminescent signal upon addition of the Detection Reagent.
GTPase, GAP and GEF activity is inversely correlated to the amount of light produced. A highly active GTPase hydrolyzes more GTP, reducing the amount of ATP produced from GTP and reducing light output. A less active GTPase hydrolyzes less GTP, leaving a larger amount of GTP to be converted to ATP and producing more light.
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
| rGTP | P115A | 1 × 50μl | 10mM | View Product |
| DTT | P117A | 1 × 100μl | 100mM | View Product |
| GTPase/GAP Buffer | V763A | 1 × 5ml | ||
| GEF Buffer | V764A | 1 × 5ml | ||
| GTPase-Glo™ Buffer | V765A | 1 × 5ml | ||
| GTPase-Glo™ Reagent, 500X | V766A | 1 × 15μl | ||
| Detection Reagent | V767A | 1 × 10ml | ||
| ADP, 10mM | V916A | 1 × 500μl |
