Description
The CD28 Blockade Bioassay is a bioluminescent cell-based assay used to measure the potency and stability of ligands or antibodies that bind and block CD28/B7 family members, CD80 and CD86 (collectively referred to as B7 for this bioassay). CD28 binds to B7 on antigen-presenting cells (APCs). Co-stimulation of T cells by CD28 activation initiates signaling cascades that result in AP-1 and NFκB transcription factor activation and nuclear translocation. These pathways significantly enhance T cell cytokine production—specifically, interleukin-2 (IL-2)—which promotes T cell proliferation, differentiation and survival.
Blockade of CD28 has proven beneficial in preclinical and clinical studies to reduce autoimmunity and alloimmunity. The CD28 Blockade Bioassay reflects the mechanism of action (MOA) of biologics designed to block the interaction of CD28 with its B7 family ligands.
Features – Benefits
- 96- and 384-well plate compatible
- Prequalified according to ICH guidelines
- No primary cell culture required
- Available in convenient thaw-and-use format
| Product | Size | Catalog |
| CD28 Blockade Bioassay | 1 × Each Component | JA6101 |
| CD28 Blockade Bioassay | 5 × Each Component | JA6105 |
| CD28 Blockade Bioassay |
Cell Propagation Model | JA7072 |
| CD28 Blockade Bioassay |
Control Ab | K1231 |
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