Description
JM109 is a K strain that is recA– and endA– to minimize recombination and improve the quality of plasmid DNA. In addition, the cells carry the F´ episome, which allows blue/white screening. JM109 Competent Cells are available for convenient transformation in two efficiencies: High Efficiency at greater than 108cfu/μg for Cloning and Single Use, and Subcloning Efficiency at greater than 107cfu/μg.
For researchers doing more than one transformation, competent cells are available in standard format (200µl aliquots). For added convenience, single-use competent cells (50µl aliquots) also are offered.
JM109 Competent Cells are prepared according to a modified procedure of Hanahan.
JM109 Genotype: endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, Δ( lac-proAB), [F´ traD36, proAB, laqIqZΔM15].
Features – Benefits
- Cells are ready to use—no preparation time necessary
- Transformation efficiencies guaranteed
- No worries about undesirable recombination events (recA– mutation), and no nuclease carryover in miniprep DNA (endA– mutation)
| Product | Size | Brand | Catalog |
| JM109 Competent Cells (Single Use) | 1 ml (20 × 50μl) | Promega | L2005 |
| JM109 Competent Cells (Cloning) | 1 ml (5 × 200μl) | Promega | L2001 |
| JM109 Competent Cells (Subcloning) |
1 ml (5 × 200μl) | Promega | L1001 |
Specifications
| Product | Item | Size | Catalog |
| JM109 Competent Cells (Single Use) | Single-Use JM109 Competent Cells, >108cfu/µg | 20 × 50μl | L200B |
| JM109 Competent Cells (Cloning) | Competent Cells Control DNA | 1 × 30μl | L107A |
| JM109 Competent Cells, High Efficiency | 5 × 200μl | L200A | |
| JM109 Competent Cells (Subcloning) | JM109 Competent Cells, Subcloning Efficiency | 5 × 200μl | L100A |
| Competent Cells Control DNA | 1 × 30μl | L107A |
