Description
Lumit™ Anti-Tag protein-protein interaction (PPI) immunoassay is a homogeneous (no-wash) assay system to measure protein-protein or protein-small molecule interactions involving tagged proteins.
The assay combines immunodetection and NanoLuc® Binary Technology (NanoBiT). NanoBiT is a structural complementation reporter ideal for interaction studies. The NanoBiT® System is composed of two subunits: Large BiT (LgBiT; 18kDa) and Small BiT (SmBiT; 11 amino acid peptide). These subunits can be expressed as recombinant fusions or chemically conjugated to antibodies or other target proteins of interest. The LgBiT and SmBiT subunits have been optimized for stability and minimal self-association due to their weak affinity (Kd = 190µM). When LgBiT and SmBiT are brought into proximity, they form a functional enzyme that generates luminescence in the presence of its substrate.
More Information :
Solution-Based Approach to Detect Protein Interactions of Tagged Proteins
- Fast and sensitive detection of protein:protein interactions in 30–120 minutes
- No wash, transfer or immobilization steps
- Reagents are anti-tag antibodies (and streptavidin) that are conjugated with Lumit™ LgBiT and SmBiT
- Tags include 6His, GST, FLAG®, human IgG and biotin
- Application Notes available below
- Available as early access material; please contact us for more information
