M-MLV Reverse Transcriptase, Rnase H minus

Description

Description

Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT [H–]), is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). This form of M-MLV Reverse Transcriptase has been genetically altered to remove the associated RNase H activity. Although many researchers are successful in using M-MLV RT (H+) for analytical and some preparative cDNA applications, reverse transcriptases lacking RNase H activity provide another option to prepare long cDNAs and libraries containing a high percentage of full-length cDNA.

Features – Benefits

• RNase H Minus: Provides optimal conditions to prepare full-length cDNA from long RNA templates.

• Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl2, 50mM DTT.
• May Be Heat-Inactivated: M-MLV RT is inactivated by heating at 70°C for 10 minutes.

Applications

• First-strand cDNA synthesis.

• Primer extension.