MagneGST™ Protein Purification System

Description

The MagneGST™ Protein Purification System provides a simple, rapid and reliable method for the purification of glutathione-S-transferase (GST) fusion proteins. Immobilized glutathione paramagnetic particles (MagneGST™ Particles) are used to isolate GST-tagged protein directly from a crude or cleared lysate using either a manual or automated procedure and requires use of a magnetic stand. GST-tagged proteins can be purified on a small scale from 1ml of culture or on a large scale using more than 50ml of culture. Samples also can be processed using a robotic platform. MagneGST™ particles are supplied as a 25% slurry and have a binding capacity of 5–10mg of GST protein per 1ml of settled resin.

Features – Benefits

• Simple : One-step purification of multiple samples with easy handling.
• Quick : After cell lysis, no requirement for high-speed centrifugation to clear lysate.
• Scalable : Scalable protocol using 1–50ml of cell culture.
• Efficient : Achieve high yields with little or no nonspecific background.

Applications

• Protein purification of glutathione-S-transferase fusion proteins from cleared or crude E. coli cell lysate.
• High-throughput purification of GST-tagged proteins using automated platforms.