Pfu DNA Polymerase

Description

Pfu DNA Polymerase is a high-fidelity, thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides in the 5´→3´ direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3´→5´ exonuclease (proofreading) activity. Base misincorporations that may occur during polymerization are rapidly excised by this proofreading activity. Consequently, Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high-fidelity synthesis. Pfu DNA Polymerase-generated PCR fragments are blunt-ended.

• 10X Reaction Buffer with MgSO4: 200mM Tris-HCl (pH 8.8 at 25°C), 100mM KCl, 100mM (NH4)2SO4, 20mM MgSO4, 1.0% Triton® X-100 and 1mg/ml nuclease-free BSA.
• Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTPs into acid-insoluble material in 30 minutes at 75°C.
• Source: Pyrococcus furiosus strain Vc1 DSM3638.
• Storage Buffer: 50mM Tris-HCl (pH 8.2 at 25°C), 0.1mM EDTA, 1mM DTT, 50% glycerol and 0.05% CHAPS.

Features – Benefits

• Low error rate thermostable DNA polymerase
• Provided with 10X buffer containing 20mM MgSO4
• Recommended for applications that require high accuracy base insertion
• Supplied at a concentration of 2–3u/μl

Specifications