pH6HTN and pH6HTC His6HaloTag® T7 Vectors

Description

The pH6HTN and pH6HTC His₆HaloTag® T7 Vectors are used for inducible expression of polyhistidine-HaloTag® fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Expression levels depend highly on the nature of the protein, but in general the N-terminal HaloTag® fusion protein can increase expression level, enhance refolding and boost solubility of the expressed protein. HaloTag® vectors are supplied as multiple cloning site (MCS) vectors for traditional cloning.

pH6HTN His₆HaloTag® T7 Vector (Cat.# G7971) is designed for protein expression with an N-terminal His₆-HaloTag® dual tag; pH6HTC His₆HaloTag® T7 Vector (Cat.# G8031) is designed for protein expression with a C-terminal His₆-HaloTag® dual tag. By coupling the protein solubility and labeling benefits of HaloTag® technology with the reusability and the throughput of Ni-affinity technology, the dual tag enables protein purification with the reusable Ni-resin while retaining the HaloTag® covalent binding with HaloTag® ligands.

Features – Benefits

• Choose from N- and C-terminal fusion options
• Use either the polyhistidine tag or HaloTag® ORF for protein expression and analysis
• Clone an protein coding region using PCR or restriction enzyme digestion

Specifications