QuantiFluor® dsDNA System

Description

The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution. The system is suitable for use in many molecular biology applications, including next-gen sequencing and other techniques where accurate DNA quantitation is important. This dye shows minimal binding to single stranded DNA (ssDNA), RNA, protein and interfering compounds, allowing specific quantitation of dsDNA.

Features – Benefits

• Experience Minimal Binding: Highly specific to dsDNA; minimal binding to ssDNA, RNA, protein and interfering compounds.
• Increase Your Sensitivity: Significantly increased sensitivity compared to absorbance at 260nm (NanoDrop® spectrophotometer) for low-concentration samples. Performs better or equal to PicoGreen® dye and can detect as little as 50pg/ml.
• Set Up Quickly and Easily: System includes all required reagents to quickly set up and quantitate dsDNA.
• Use with Promega Instruments: Pre-optimized on both the Quantus™ Fluorometer and GloMax®-Multi+ Instrument.
• Use for Next-Gen Sequencing: Successfully used in several Next-Gen Sequencing systems, including Illumina (HiSeq/miSeq), Roche (454) and LifeTech (ion Torrent and ion Proton) systems.

Applications

• Quantification of double-stranded DNA using fluorescent methods.
• Quantification of dsDNA prior to next-gen sequencing (see citations).

Specifications

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