Description
The Reverse Transcription System provides reagents to efficiently reverse transcribe RNA into cDNA in 15 minutes. The cDNA prepared from each reaction using this system may be used directly in multiple PCR amplifications using Taq DNA polymerase. The AMV Reverse Transcriptase synthesizes single-stranded cDNA from total or poly(A)+ RNA. Both Oligo(dT)15 and Random Primers are included, allowing cDNA synthesis from virtually any RNA source. The system contains sufficient reagents for 100 cDNA synthesis reactions, processing 1μg of RNA per reaction. Each cDNA synthesis reaction may be divided and used in up to 20 separate PCR amplifications. A polyadenylated 1.2kb RNA transcript is provided as a control template for cDNA synthesis.
Features – Benefits
- Speed: Efficiently reverse transcribe poly(A)+ mRNA or total RNA in 15 minutes.
- Convenience: PCR-compatible components are provided in optimized volumes for 100 reactions.
- Positive Controls: A polyadenylated RNA transcript is provided to help troubleshoot RT-PCR parameters.
| Product | Size | Brand | Catalog |
| Reverse Transcription System | 100 reactions | Promega | A3500 |
Specifications
| Item | Size | Catalog |
| MgCl2 1,5 mL (25 mM) | 1 × 1.2ml | A351H |
| Reverse Transcription 10X Buffer | 1 × 1.4ml | A356A |
| Oligo(dT)15 Primer 500μg/ml | 1 × 50μg | C110B |
| dNTP Mix 10 mM | 1 × 320μl | C114B |
| Random Primers | 1 × 50μg | C118B |
| 1.2kb Kanamycin Positive Control RNA (0.5 mg/ml) | 1 × 5μg | C138A |
| AMV Reverse Transcriptase (HC) | 1 × 1,500u | M900B |
| Recombinant RNasin® Ribonuclease Inhibitor (40u/μl) | 1 × 2,500u | N251A |
| Nuclease-Free Water | 1 × 13ml | P119E |
