Stains & Dyes

Description

SILVER STAINING-DESTAINING KIT

Overview

• Ultra-sensitive protein detection with clear background
• Liquid concentrates for convenient reagent preparation
• Mass spectrometry compatible

Detailed Description

The Silver Staining – Destaining Kit is a combination of two products for convenience; Silver Bullit™ Silver Stain Kit (for 50 mini-gels) and Silver Subtract™ Silver Destaining Reagent (for 12 mini-gels). Proteins can be detected in polyacrylamide gels with high sensitivity and nearly undetectable background using AMRESCO’s Silver Bullit™ Silver Stain Kit. The kit’s colorimeteric staining procedure allows detection of subnanogram levels of protein, and is 100-fold more sensitive than Coomassie® Blue staining. This product is ideal for visualization of proteins present in trace levels on a gel.

Gels stained with Silver Bullit™ or any other silver staining product can be completely destained with Silver Subtract™ Silver Destaining Reagent. It is an excellent kit for the removal of silver ions before re-staining with silver stain. Silver Subtract™ is also compatible with subsequent visualization of the gel with with fluorescent or chemiluminescent stains, or with chromogenic stains such as Coomassie® Blue. Partial destaining can also be achieved with less dilute reagents to remove high background, reduce staining of overloaded gels, or remove artifacts and uneven background. Gels re-stained after using Silver Subtract™ do not exhibit a decrease in band intensity or increase in background.