Description
TMB Stabilized Substrate is a stable, ready-to-use TMB (3,3´, 5,5´-tetramethylbenzidine) color development substrate for localization of horseradish peroxidase-conjugated antibodies on dot blots and Western blots. It is easier to use than 4-chloro-1-naphthol (CN), which must be prepared immediately before use. TMB Stabilized Substrate comes premixed and fully diluted in a proprietary buffer containing less than 0.5% organic solvent.
TMB Stabilized Substrate is at least threefold more sensitive than 4-chloro-1-naphthol (CN); as little as 412pg of β-galactosidase detected on TMB blot as compared to 1.12ng on CN blot when detected with a β-galactosidase-specific antibody and HRP-conjugated secondary antibody. The color is much more stable than 4-chloro-1-naphthol and photographs more easily.
Features
- Premixed and ready to use
- Stable at room temperature for 12 months
- More sensitive and color-stable than 4-chloro-1-naphthol
| Size | Cat.No. |
| 200ml | W4121 |
