UMP/CMP-Glo™ Glycosyltransferase Assay

Description

The GDP-Glo™ Glycosyltransferase Assay is a homogeneous, single-reagent-addition method to rapidly detect GDP formation in glycosyltransferase reactions. After the glycosyltransferase reaction, an equal volume of GDP Detection Reagent is added to simultaneously convert the GDP product to ATP and generate light in a luciferase reaction. The light generated is detected using a luminometer, and the light output is proportional to the concentration of GDP from low nM to 25μM. Luminescence can be correlated to GDP concentration by using a GDP standard curve.

Features – Benefits

• Easy, “add-incubate-read” protocol
• Detects sialyltransferases and phosphoglycosyltransferases that use CMP-, CDP- or UDP-sugars as donor substrates
• Ideal for low-activity glycosyltransferases

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