Description
Description
The Wizard® SV 96 and SV 9600 Plasmid DNA Purification Systems provide a simple and reliable method for the rapid isolation of plasmid DNA using a silica-membrane, 96-well, high-throughput format. A single plate can be processed in 60 minutes or less. The Vac-Man® 96 Vacuum Manifold eliminates waste handling and allows simultaneous lysate clearing and DNA binding, and the plate design prevents cross-contamination during sample processing.
Purified plasmid can be used directly for automated fluorescent DNA sequencing as well as for other standard molecular biology techniques. Purified DNA meets a target of >98% accuracy over 600 bases using pGEM®-3Zf(+) Vector DNA in BigDye® terminator sequencing.
The Wizard® SV 96 and SV 9600 Systems are designed for use either in a manual format or with Beckman Coulter or PerkinElmer automated instruments.
The Wizard® SV 96 and Wizard® SV 9600 Systems require a vacuum pump capable of generating 15–20 inches of Hg or equivalent and the Vac-Man® 96 Vacuum Manifold (Cat.# A2291) or similar manifold. The Vac-Man® 96 Vacuum Manifold requires the use of an external vacuum trap (not provided).
Features – Benefits
- 60-minute protocol
- Manual and automated formats
- Vacuum-based method
Product | Size | Brand | Catalog |
Wizard® SV 96 and SV 9600 Plasmid DNA Purification Systems | 1 x 96 preps | Promega | A2250 |
5 x 96 preps | A2255 | ||
100 x 96 preps | A2258 |