Reporter Assays

A bioluminescent reporter assay consists of both a luciferase reporter enzyme and a detection reagent that provides the enzyme substrate. When the reporter enzyme and detection reagent are combined, the light emitted is proportional to reporter gene expression levels and is detected using a luminometer. We offer bioluminescent reagents for detecting NanoLuc®, firefly, and Renilla luciferases, with multiple detection reagent options for each enzyme to create a reporter assay optimized for specific experimental goals. The detection reagents have varying signal brightness and stability, include both lytic or live-cell options, and can provide either single- or dual-reporter detection.

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