cAMP-Glo™ Max Assay

Description

This improved version of the cAMP-Glo™ Assay combines the lysis and cAMP reaction buffers into the cAMP-Glo™ ONE Buffer, streamlining the protocol and speeding up the assay. The ONE Buffer is supplied at 5X concentration, which provides increased flexibility for starting cell culture volumes.

The cAMP-Glo™ Max Assay allows easy quantitation of cAMP levels in broad range of cell types, and can be performed in 96-, 384- or 1536-well plates. Cells are induced with a test compound for an appropriate period of time to modulate cAMP levels. After induction, cells are lysed and the cAMP released stimulates protein kinase A in the reagent. The Kinase-Glo® Reagent is then added to terminate the PKA reaction and detect the remaining ATP via a luciferase reaction. Plates are read using a microplate-reading luminometer. The half-life for the luminescent signal is greater than 4 hours, providing ample time to read plates and eliminating the need for luminometers with reagent injectors.

Features – Benefits

• Fast and Easy to Use:
  • Assay can be completed in approximately 30 minutes.
  • Improved—Lysis and cAMP detection steps combined (cAMP-Glo™ ONE Buffer).
  • ONE Buffer—5X concentration provides better flexibility for starting cell culture volumes.
• Excellent Signal-to-Noise Ratios:
  • Best signal:background ratio of all the cAMP assays.
  • Signal:Background >200 (with cAMP), >15 (on cells).
• Proven Luminescent Technology:
  • Powered by Ultra-Glo™ Recombinant Luciferase.
  • No interference by fluorescent compounds.
  • Non-radioactive.

For more specifications, click here