GoTaq® G2 Polymerase and Master Mixes

Description

The second generation of GoTaq® products, GoTaq® G2 DNA Polymerase reliably amplifies a wide range of PCR templates and provides high-performing results due to improved manufacturing processes, increased reliability and consistency. The product is available in many formats to give you maximum flexibility, control and convenience for your PCR. For robust, routine PCR choose a standalone enzyme and buffer system with or without magnesium. For maximum convenience choose an all-in-one master mix.

For superior convenience with hot-start PCR see GoTaq® G2 Hot Start Polymerase.

GoTaq® G2 DNA Polymerase is supplied with 5X Green GoTaq® Reaction Buffer and 5X Colorless GoTaq® Reaction Buffer. Both buffers contain MgCl₂ at a concentration of 7.5mM for a final concentration of 1.5mM in the 1X reaction.

GoTaq® G2 Flexi DNA Polymerase is supplied with 5X Green GoTaq® Flexi Buffer and 5X Colorless GoTaq® Flexi Buffer and 25mM MgCl₂.

GoTaq® G2 Green and Colorless Master Mixes are ready-to-use, green and colorless, 2X master mixes containing all necessary components for robust, reliable PCR, including the second-generation GoTaq® G2 DNA Polymerase. Add template, primers and go.

The GoTaq® G2 and G2 Flexi DNA Polymerases are supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification. The enzyme is a full-length form of Taq DNA polymerase that exhibits 5´→3´ exonuclease activity. The 5X Green GoTaq® Reaction and Flexi Buffers contain two dyes (blue and yellow) that separate during electrophoresis to indicate migration progress. The colorless buffer is used when direct fluorescence or absorbance readings are required without prior purification of the amplified DNA from the PCR.