Description
GoTaq® DNA Polymerase is a proprietary formulation of Taq DNA polymerase that gives robust amplification equal to and, in some cases, superior to that of standard Taq DNA polymerase.
For ultimate in convenience, choose GoTaq® Colorless Master Mix or GoTaq® Green Master Mix and directly substitute in your current PCR application—no need to change cycling parameters.
Both Master Mixes are ready-to-use 2X solutions that contain GoTaq® DNA Polymerase, dNTPs, MgCl2 and reaction buffer at optimal concentrations for efficient amplification of DNA templates by PCR. GoTaq® Green Master Mix also includes two dyes (blue and yellow) that allow monitoring of progress during electrophoresis. The blue dye comigrates at the same rate as 3–5kb DNA fragments in a 1% agarose gel. The yellow dye migrates ahead of primers (<50bp).
GoTaq® Colorless Master Mix has the same formulation as the GoTaq® Green Master Mix but does not include the dyes. Both also include Nuclease-Free Water. Reactions assembled with the GoTaq® Master Mixes have sufficient density for direct loading onto agarose gels.
Reactions can be set up in less than a minute; simply add your template, water and primers and go!
GoTaq® Green and Colorless Master Mix reaction size is based on a 50µl reaction volume.
Features – Benefits
- Directly substitute into your current PCR conditions
- Use the green master mix for direct-to-gel analysis after amplification
- Use the colorless master mix for post-amplification analysis by fluorescence or absorbance without prior DNA purification
Product | Size | Brand | Catalog |
GoTaq® Master Mixes (colorless) |
100 reactions | Promega | M7132 |
1000 reactions | Promega | M7133 | |
GoTaq® Master Mixes (green) |
100 reactions | Promega | M7122 |
1000 reactions | Promega | M7123 |
Specifications
Product | Item | Size | Catalog |
GoTaq® Master Mixes (colorless) 1000 reactions | GoTaq® Colorless Master Mix, 2X | 1 × 25ml | M713C |
Nuclease-Free Water | 1 × 25ml | P119C | |
GoTaq® Master Mixes (colorless) 100 reactions | GoTaq® Colorless Master Mix, 2X | 2 × 1,250μl | M713B |
Nuclease-Free Water | 2 × 1,250μl | P119A | |
GoTaq® Master Mixes (green) 1000 reactions |
GoTaq® Green Master Mix, 2X | 1 × 25ml | M712C |
Nuclease-Free Water |
1 × 25ml | P119C | |
GoTaq® Master Mixes (green) 100 reactions |
GoTaq® Green Master Mix, 2X | 2 × 1,250μl | M712B |
Nuclease-Free Water |
2 × 1,250μl | P119A |