Description
The GoTaq® RT-qPCR Systems are ready-to-use, 2X master mixes containing BRYT Green® Dye, a fluorescent DNA binding dye with minimal PCR inhibition, providing maximum amplification efficiency and greater fluorescence enhancement than SYBR® Green I. Rapid hot-start activation and processive enzymes make the GoTaq® Systems compatible with most real time PCR instruments using standard or fast cycling. The GoTaq® 1-Step qPCR Master Mix and GoTaq® 2-Step qPCR Master Mix also include GoScript™ Reverse Transcriptase to provide efficient synthesis of first strand cDNA in preparation for PCR.
GoTaq® RT-qPCR System has two categories which are GoTaq® 1-Step RT-qPCR System and GoTaq® 2-Step RT-qPCR System. The GoTaq® 1-Step RT-qPCR and GoTaq® 2-Step RT-qPCR provide detection and relative quantification of RNA expression levels using GoScript™ Reverse Transcriptase and GoTaq® qPCR Master Mix. The GoTaq® qPCR Master Mix consists of GoTaq® Hot Start Polymerase, MgCl2, dNTPs and BRYT Green® Dye and a proprietary reaction buffer. The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities amounts of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions.
Features and Benefits
- Ready to use mix of optimized components for dye-based qPCR and RT-qPCR.
- Compatible with both fast and standard cycling methods.
- Resistant to a wide range of PCR inhibitors.
- Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range. Detects down to femtogram (fg) amounts of input RNA.
- Exceptional room-temperature setup, allowing easy automation and high-throughput detection.
Product | Size | 10µl Reactions | 20µl Reactions | Brand | Catalog |
GoTaq® 1-Step RT-qPCR System | 5 ml | 1000 reactions | 500 reactions | Promega | A6020 |
GoTaq® 2-Step RT-qPCR System | 5 ml | 5000 reactions | 2500 reactions | Promega | A6010 |
Specifications GoTaq® 1-Step RT-qPCR System
Item | Size | Catalog |
MgCl2 (25 mM) | 1 × 750μl | A351B |
GoScript™ RT Mix for 1-Step RT-qPCR (50X) | 1 × 225μl | M700A |
GoTaq® qPCR Master Mix, 2X | 5 × 1ml | A600A |
CXR Reference Dye (30μM) | 1 × 200μl | C541B |
Nuclease-Free Water | 2 × 13ml | P119E |
Specifications GoTaq® 2-Step RT-qPCR System
Item | Size | Catalog |
MgCl2 (25 mM) | 1 × 750μl | A351B |
GoScript™ 5X Reaction Buffer | 1 × 300μl | A500B |
GoScript™ Reverse Transcriptase | 1 × 50μl | A501B |
GoTaq® qPCR Master Mix, 2X | 5 × 1ml | A600A |
Oligo(dT)15 Primer (500μg/ml) | 1 × 50μg | C110B |
PCR Nucleotide Mix (10 mM) | 1 × 200μl | C114G |
Random Primers | 1 × 50μg | C118B |
CXR Reference Dye | 1 × 100μl | C541A |
Recombinant RNasin® Ribonuclease Inhibitor (40u/μl) | 1 × 2,500u | N251A |
Nuclease-Free Water | 2 × 13ml | P119E |
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