GoTaq® RT-qPCR Systems

The GoTaq® 1-Step qPCR Master Mix and GoTaq® 2-Step qPCR Master Mix also include GoScript™ Reverse Transcriptase to provide efficient synthesis of first strand cDNA in preparation for PCR.

Description

The GoTaq® RT-qPCR Systems are ready-to-use, 2X master mixes containing BRYT Green® Dye, a fluorescent DNA binding dye with minimal PCR inhibition, providing maximum amplification efficiency and greater fluorescence enhancement than SYBR® Green I. Rapid hot-start activation and processive enzymes make the GoTaq® Systems compatible with most real time PCR instruments using standard or fast cycling. The GoTaq® 1-Step qPCR Master Mix and GoTaq® 2-Step qPCR Master Mix also include GoScript™ Reverse Transcriptase to provide efficient synthesis of first strand cDNA in preparation for PCR.

GoTaq® RT-qPCR System has two categories which are GoTaq® 1-Step RT-qPCR System and GoTaq® 2-Step RT-qPCR System. The GoTaq® 1-Step RT-qPCR and GoTaq® 2-Step RT-qPCR provide detection and relative quantification of RNA expression levels using GoScript™ Reverse Transcriptase and GoTaq® qPCR Master Mix. The GoTaq® qPCR Master Mix consists of GoTaq® Hot Start Polymerase, MgCl2, dNTPs and BRYT Green® Dye and a proprietary reaction buffer. The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities amounts of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions.

Features and Benefits

  • Ready to use mix of optimized components for dye-based qPCR and RT-qPCR.
  • Compatible with both fast and standard cycling methods.
  • Resistant to a wide range of PCR inhibitors.
  • Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range. Detects down to femtogram (fg) amounts of input RNA.
  • Exceptional room-temperature setup, allowing easy automation and high-throughput detection.
Product Size 10µl Reactions 20µl Reactions Brand Catalog
GoTaq® 1-Step RT-qPCR System 5 ml 1000 reactions 500 reactions Promega A6020
GoTaq® 2-Step RT-qPCR System 5 ml 5000 reactions 2500 reactions Promega A6010

Specifications GoTaq® 1-Step RT-qPCR System

Item Size Catalog
MgCl2 (25 mM) 1 × 750μl A351B
GoScript™ RT Mix for 1-Step RT-qPCR (50X) 1 × 225μl M700A
GoTaq® qPCR Master Mix, 2X 5 × 1ml A600A
CXR Reference Dye (30μM) 1 × 200μl C541B
Nuclease-Free Water 2 × 13ml P119E

Specifications GoTaq® 2-Step RT-qPCR System

Item Size Catalog
MgCl2 (25 mM) 1 × 750μl A351B
GoScript™ 5X Reaction Buffer 1 × 300μl A500B
GoScript™ Reverse Transcriptase 1 × 50μl A501B
GoTaq® qPCR Master Mix, 2X 5 × 1ml A600A
Oligo(dT)15 Primer (500μg/ml) 1 × 50μg C110B
PCR Nucleotide Mix (10 mM) 1 × 200μl C114G
Random Primers 1 × 50μg C118B
CXR Reference Dye 1 × 100μl C541A
Recombinant RNasin® Ribonuclease Inhibitor (40u/μl) 1 × 2,500u N251A
Nuclease-Free Water 2 × 13ml P119E

 

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Additional information

Brand

Promega