Description
The GoTaq® Probe qPCR are ready-to-use 2X master mixes that simplify reaction assembly for qPCR using hydrolysis probe detection. These real-time qPCR systems are designed for sensitive detection and quantification of a broad range of DNA or RNA targets in the presence of a wide range of PCR inhibitors.
Rapid hot-start activation and processive enzymes make the GoTaq® Probe qPCR compatible with most real-time PCR instruments using standard or fast cycling. Convenient, room-temperature setup makes it easy to scale up to automated and high-throughput detection formats.
The GoTaq® Probe qPCR Master Mix is a ready-to-use, stabilized, 2X formulation with all of the components for quantitative PCR (qPCR). Just add template, primers and probe. Optimized for fast and reproducible quantitative PCR assays in the hydrolysis probe detection format, the master mix contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs and a proprietary reaction buffer. The master mix does not contain a reference dye; a separate tube of CXR reference dye is included.
Features and Benefits:
- Ready to use mix of optimized components for robust qPCR and RT-qPCR using hydrolysis probes.
- Compatible with fast and standard cycling methods on most real-time PCR instruments using TaqMan® and other probe assays such as molecular beacons.
- Sensitive detection for earlier quantitation of low- and high- copy targets.
- Optimized formulation compatible with running multiplex assays.
- Room temperature setup, making it suitable for automation and high throughput detection.
- Resistance to a wide range of PCR inhibitors.
Product | Size | 10µl Reactions | 20µl Reactions | Brand | Catalog |
GoTaq® Probe qPCR Master Mix | 2 ml | 400 reactions | 200 reactions | Promega | A6101 |
GoTaq® Probe qPCR Master Mix | 10 ml | 2000 reactions | 1000 reactions | Promega | A6102 |
Specifications
Item | Size | Catalog |
GoTaq® qPCR Master Mix, 2X | 2 × 1 ml | A610A |
CXR Reference Dye | 1 × 100μl | C541A |
Nuclease-Free Water | 2 × 1,250μl | P119A |