Description
The HDAC-Glo™ and SIRT-Glo™ Assays and Screening Systems provide a simple “Add-Mix-Measure” method that achieves maximum signal in as little as 15 minutes and is 10-to 100X more sensitive than comparable fluorescence-based systems. The HDAC-Glo™ I/II Assays and Screening Systems are single-reagent-addition, homogeneous, luminescent assays that measure the relative activity of histone deacetylase (HDAC) class I and II enzymes from cells, extracts or purified enzyme sources. The assays use an acetylated, live-cell-permeant, luminogenic peptide substrate that can be deacetylated by HDAC activities. Deacetylation of the peptide aminoluciferin substrate is measured using a coupled enzymatic system in which a protease in the Developer Reagent cleaves the deacetylated peptide from the aminoluciferin substrate, releasing aminoluciferin, which is quantified in a reaction using Ultra-Glo™ recombinant firefly luciferase. The assay reaction is typically complete within 15–45 minutes with no sample manipulation. The HDAC-mediated luminescent signal is persistent, with a half-life of greater than 3 hours, allowing batch processing of multiwell plates. The HDAC assay is broadly useful for class I and II enzymes.
Applications
- Determine HDAC inhibitor potency using purified enzymes, extracts or cells directly in culture plates.
- Profile HDAC inhibitors with purified enzymes.
- Correlate HDAC inhibitor potency with cellular fate in same-well multiplexed viability assays.
- Confirm deacetylase inhibition with control substrates.
| PRODUCT | SIZE | BRAND | CATALOG |
| HDAC-Glo™ I/II Assay | 10 ml | Promega | G6420 |
| HDAC-Glo™ I/II Assay | 5 x 10 ml | Promega | G6421 |
| HDAC-Glo™ I/II AssayThe | 100 ml | Promega | G6422 |
| HDAC-Glo™ I/II Screening System | 10 ml | Promega | G6430 |
| HDAC-Glo™ I/II Screening System | 5 x 10 ml | Promega | G6431 |
There are no reviews yet.