Description
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71kDa. The RNase H activity of M-MLV RT is weaker than that of the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase.
Features – Benefits
- Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl2, 50mM DTT.
- May Be Heat-Inactivated: M-MLV RT is inactivated by heating at 70°C for 10 minutes.
| Product | Size | Brand | Catalog |
| M-MLV Reverse Transcriptase (Enzyme) | 10000u | Promega | M1701 |
| M-MLV Reverse Transcriptase (Enzyme) | 50000u | Promega | M1705 |
| M-MLV Reverse Transcriptase (Buffer Pack) | 2 × 1 ml | Promega | M5313 |
Specifications
| Product | M-MLV Reverse Transcriptase | M-MLV RT 5X Buffer | ||
| Size | Catalog | Size | Catalog | |
| M-MLV Reverse Transcriptase (Enzyme) 10000u | 1 × 10000u | M170A | 1 × 1 ml | M531A |
| M-MLV Reverse Transcriptase (Enzyme) 50000u | 1 × 50000u | M170B | 2 × 1 ml | M531A |
| M-MLV Reverse Transcriptase (Buffer Pack) 2 × 1 ml | – | – | 2 × 1 ml | M531A |
