Description
The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3´ of the firefly luciferase gene (luc2). Firefly luciferase is the primary reporter gene; reduced firefly luciferase expression indicates the binding of endogenous or introduced miRNAs to the cloned miRNA target sequence. This vector is based on Promega dual-luciferase technology, with luc2 used as the primary reporter to monitor mRNA regulation and Renilla luciferase (hRluc-neo) acting as a control reporter for normalization and selection.
Features – Benefits
- Measure miRNA Function: Reporter activity correlates with miRNA activity.
- Optimized Reporter Genes: luc2 gene provides highest expression.
- Combination Renilla/Neomycin Marker: Normalize with Dual-Luciferase® Assay or for stable cell lines, all with one vector.
- Biologically Relevant Results: The moderate-strength PGK promoter provides sensitive analysis not possible with strong promoters.
Applications
- miRNA detection.
- miRNA binding site analysis.
- Transcript stability study.
- 3´-UTR analysis.
Product | Size | Brand | Catalog |
pmirGLO Dual-Luciferase miRNA Target Expression Vector | 20μg | Promega | E1330 |
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