pmirGLO Dual-Luciferase miRNA Target Expression Vector

Description

The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3´ of the firefly luciferase gene (luc2). Firefly luciferase is the primary reporter gene; reduced firefly luciferase expression indicates the binding of endogenous or introduced miRNAs to the cloned miRNA target sequence. This vector is based on Promega dual-luciferase technology, with luc2 used as the primary reporter to monitor mRNA regulation and Renilla luciferase (hRluc-neo) acting as a control reporter for normalization and selection.

Features – Benefits

• Measure miRNA Function: Reporter activity correlates with miRNA activity.
• Optimized Reporter Genes: luc2 gene provides highest expression.
• Combination Renilla/Neomycin Marker: Normalize with Dual-Luciferase® Assay or for stable cell lines, all with one vector.
• Biologically Relevant Results: The moderate-strength PGK promoter provides sensitive analysis not possible with strong promoters.

Applications

• miRNA detection.
• miRNA binding site analysis.
• Transcript stability study.
• 3´-UTR analysis.

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