Description
The ProDye™ Terminator Sequencing System provides an improved method of fluorescent Sanger sequencing using a proprietary thermostable DNA polymerase included in the master mix. Compatible with a variety of DNA templates, the ProDye™ Terminator Sequencing System can be used with the Spectrum Compact CE System as well as the Applied Biosystems® 3130, 3130xl, 3500, 3500xL, 3730, 3730xl and SeqStudio® Genetic Analyzers. The ProDye™ Terminator Sequencing System uses dTMR, dCXR, dRSixG and dROneTen terminator dyes, the same dyes contained in the BigDye® Terminator V3.1 Cycle Sequencing Kit.
Features – Benefits
- Easily implemented with the same protocol, workflows and calibration
- Designed for a wide range of sequencing applications
- Provides enhanced productivity while reducing costs
| Product | Size | Brand | Catalog |
| ProDye™ Terminator Sequencing System | 24 reactions | Promega | CR4324 |
| ProDye™ Terminator Sequencing System | 200 reactions | Promega | CR4302 |
| ProDye™ Terminator Sequencing System | 1000 reactions | Promega | CR4310 |
Specifications ProDye™ Terminator Sequencing System (24 reactions)
| Item | Size | Catalog |
| ProDye™ 2.5X Master Mix | 1 × 200μl | CR100A |
| ProDye™ 5X Sequencing Buffer | 1 × 1ml | CR101A |
| pGEM®-3Zf(+) Vector | 1 × 20μg | P227A |
| pUC/M13 Primer, Forward (24mer) | 1 × 2μg | Q560A |
| Nuclease-Free Water | 1 × 1.25ml | P119A-C |
Specifications ProDye™ Terminator Sequencing System (200 reactions)
| Item | Size | Catalog |
| ProDye™ 2.5X Master Mix | 2 × 800μl | CR100B |
| ProDye™ 5X Sequencing Buffer | 3 × 1ml | CR101A |
| pGEM®-3Zf(+) Vector | 1 × 20μg | P227A |
| pUC/M13 Primer, Forward (24mer) | 1 × 2μg | Q560A |
| Nuclease-Free Water | 5 × 1,250μl | P119A |
Specifications ProDye™ Terminator Sequencing System (1000 reactions)
| Item | Size | Catalog |
| ProDye™ 2.5X Master Mix | 10 × 800μl | CR100B |
| ProDye™ 5X Sequencing Buffer | 1 × 12ml | CR101B |
| pGEM®-3Zf(+) Vector | 1 × 20μg | P227A |
| pUC/M13 Primer, Forward (24mer) | 1 × 2μg | Q560A |
| Nuclease-Free Water | 2 × 13ml | P119E |
