Kinase Target Engagement

The NanoBRET™ Target Engagement (TE) Intracellular Kinase Assays provide a way to quantitatively measure specific kinase-inhibitor interactions in live cells using Bioluminescence Resonance Energy Transfer (BRET). The assay measures the apparent cellular affinity of test compounds bound by competitive displacement of a cell-permeable fluorescent NanoBRET™ tracer, reversibly bound to a kinase-NanoLuc® luciferase fusion in cells.