Description
The AccessQuick™ RT-PCR System is an easy and convenient master mix for one-tube RT-PCR. It increases the convenience of performing RT-PCR by combining the following components in a single tube: Tfl DNA Polymerase, dNTPs, magnesium sulfate and reaction buffer. The AMV RT enzyme is provided in a separate tube to allow important no-RT control reactions. The AccessQuick™ Master Mix is simply added to RNA templates in reaction vials, followed by the AMV RT, primers and water. The AccessQuick™ RT-PCR Master Mix is intended for routine RT-PCR applications that have been previously optimized and do not require extreme conditions.
Features – Benefits
- Maximum Convenience: Save yourself four pipetting steps. Simply combine the AccessQuick™ Master Mix, AMV RT, your gene-specific primers, your RNA template and water. Separate AMV RT allows important no-RT control reactions.
- Less Template: Amplify zeptomole (10–21mol) levels of RNA.
- No Buffer Additions Required: Set up reactions in a single tube, place in the thermal cycler and come back later for results—no additions between the reverse transcription and DNA amplification steps.
- Stability: System components are stable over many freeze-thaw cycles.
Product | Size | Brand | Catalog |
AccessQuick™ RT-PCR System | 20 reactions | Promega | A1701 |
AccessQuick™ RT-PCR System | 100 reactions | Promega | A1702 |
AccessQuick™ RT-PCR System | 500 reactions | Promega | A1703 |
Specifications
Product |
Item |
|||||
AccessQuick™ Master Mix (2X) | AMV Reverse Transcriptase | Nuclease-Free Water | ||||
Size | Catalog | Size | Catalog | Size | Catalog | |
AccessQuick™ RT-PCR System 20 reactions | 2 × 250μl | A170A | 1 × 100u | A261B | 1 × 1,250μl | P119A |
AccessQuick™ RT-PCR System 100 reactions | 3 × 1ml | A170B | 1 × 500u | A261A | 2 × 1,250μl | P119A |
AccessQuick™ RT-PCR System 500 reactions | 1 × 12.5ml | A170C | 1 × 2,500u | A261C | 1 × 25ml | P119C |
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