AccessQuick™ RT-PCR System

The AccessQuick™ RT-PCR System is an easy and convenient master mix for one-tube RT-PCR. It increases convenience by combining Tfl DNA Polymerase, dNTPs, magnesium sulfate and reaction buffer in a single master mix.

 

Description

The AccessQuick™ RT-PCR System is an easy and convenient master mix for one-tube RT-PCR. It increases the convenience of performing RT-PCR by combining the following components in a single tube: Tfl DNA Polymerase, dNTPs, magnesium sulfate and reaction buffer. The AMV RT enzyme is provided in a separate tube to allow important no-RT control reactions. The AccessQuick™ Master Mix is simply added to RNA templates in reaction vials, followed by the AMV RT, primers and water. The AccessQuick™ RT-PCR Master Mix is intended for routine RT-PCR applications that have been previously optimized and do not require extreme conditions.

Features – Benefits

  • Maximum Convenience: Save yourself four pipetting steps. Simply combine the AccessQuick™ Master Mix, AMV RT, your gene-specific primers, your RNA template and water. Separate AMV RT allows important no-RT control reactions.
  • Less Template: Amplify zeptomole (10–21mol) levels of RNA.
  • No Buffer Additions Required: Set up reactions in a single tube, place in the thermal cycler and come back later for results—no additions between the reverse transcription and DNA amplification steps.
  • Stability: System components are stable over many freeze-thaw cycles.
Product Size Brand Catalog
AccessQuick™ RT-PCR System 20 reactions Promega A1701
AccessQuick™ RT-PCR System 100 reactions Promega A1702
AccessQuick™ RT-PCR System 500 reactions Promega A1703

Specifications

Product
Item
AccessQuick™ Master Mix (2X) AMV Reverse Transcriptase Nuclease-Free Water
Size Catalog Size Catalog Size Catalog
AccessQuick™ RT-PCR System 20 reactions 2 × 250μl A170A 1 × 100u A261B 1 × 1,250μl P119A
AccessQuick™ RT-PCR System 100 reactions 3 × 1ml A170B 1 × 500u A261A 2 × 1,250μl P119A
AccessQuick™ RT-PCR System 500 reactions 1 × 12.5ml A170C 1 × 2,500u A261C 1 × 25ml P119C

 

Additional information

Brand

Promega

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