AMV Reverse Transcriptase

Description

Avian Myeloblastosis Virus Reverse Transcriptase (AMV RT) catalyzes DNA polymerization using template DNA, RNA or RNA:DNA hybrids. It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg²⁺ or Mn²⁺. The enzyme possesses an intrinsic RNase H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro.

Features – Benefits

• High Concentration: Cat.# M9004 contains 600 units of AMV RT at 20–25u/μl.
• 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 250mM KCl, 50mM MgCl₂, 2.5mM spermidine, 50mM DTT.
• Temperature Stability: AMV RT is the preferred reverse transcriptase for templates with high secondary structure due to its stability at higher reaction temperatures (37–58°C).

Applications

• First- and second-strand synthesis of cDNA.
• Primer extensions and RNA sequencing.
• RT-PCR. Up to 10μl of an RT reaction containing AMV RT and the supplied AMV RT Reaction Buffer can be added to a 50μl PCR amplification reaction that uses Taq DNA polymerase. If GoTaq® DNA Polymerase or PCR Master Mix are used, up to 25μl of an RT reaction can be added per 50μl PCR.

Specifications