GoScript™ Reverse Transcription System

Description

The GoScript™ Reverse Transcription System is a convenient kit that includes a reverse transcriptase and an optimized set of reagents designed for efficient synthesis of first-strand cDNA optimized in preparation for PCR amplification. The components of the GoScript™ Reverse Transcription System can be used to reverse transcribe RNA templates starting with total RNA, poly(A)+ mRNA or synthetic transcript RNA. Figure 1 provides an overview of the reverse transcription procedure. The optimized reaction buffer and reverse transcriptase provided in the GoScript™ Reverse Transcription System enable robust, full-length cDNA synthesis for reproducible analysis of rare or long messages.

These conditions were developed for cDNA synthesis or for easy transition to gene-specific target amplification. Up to 5µl of the reverse transcription reaction can be directly amplified using Taq DNA polymerase in a 25µl PCR. GoScript™ Reverse Transcriptase is qualified for use in two-step RT-qPCR using GoTaq® qPCR and Plexor® qPCR Systems. In two-step RT-qPCR using GoTaq® qPCR Master Mix, samples of the GoScript™ Reverse Transcription System can be added directly, up to 20% v/v.

Features – Benefits

• Ultra-Active: Save money on every reaction.
• Sensitive: Detect rare transcripts.
• Processive: Transcribe long messages.
• Resilient: Synthesize cDNA in the presence of strong inhibitors.

Applications

• RT-qPCR.
• RT-PCR.

Available Separately