Description
The QuantiFluor® ONE dsDNA System contains a fluorescent double-stranded DNA-binding dye (504nmEx/531nmEm) developed for use in an “add-and-read” format for dye and standard, making sample quantitation easy. This system enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA).
The QuantiFluor® ONE dsDNA System was developed using the fluorescence module of the GloMax® Multi+ Detection System with Instinct® Software, GloMax® Discover System and the Quantus™ Fluorometer. The QuantiFluor® ONE dsDNA System can be used with any fluorometer that is capable of measuring fluorescence at the appropriate excitation and emission wavelengths.
Features – Benefits
- Perform No Dilutions; Use No Extra Tubes : Add-and-read format makes this dye simple to use.
- Increase Your Sensitivity: Significantly increased sensitivity compared to absorbance at 260nm (NanoDrop® spectrophotometer), allowing you to quantitate low-concentration samples with confidence.
- Experience Minimal Binding: Highly specific to dsDNA; minimal binding to ssDNA, RNA, protein and interfering compounds.
- Take Advantage of Flexible Instrumentation: Integrated on Quantus™ and GloMax® detection instruments, yet compatible with any fluorometer capable of measuring the appropriate fluorescence excitation and emission spectra.
Applications
- dsDNA quantitation.
| Product | Size | Brand | Catalog |
| QuantiFluor® ONE dsDNA System | 100 reactions | Promega | E4871 |
| QuantiFluor® ONE dsDNA System | 500 reactions | Promega | E4870 |
Specifications
| Item | Size | Catalog |
| QuantiFluor® ONE dsDNA Dye | 1 × 20ml | E489A |
| QuantiFluor® ONE Lambda DNA | 1 × 80μg | E490B |
| 1X TE Buffer (pH 7.5) | 1 × 10ml | E497B |
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