Description
The Maxwell® HT 96 gDNA Blood Isolation System provides a simple and reliable method for rapid isolation of genomic DNA in a multiwell format. gDNA may be purified from blood and Oragene®•Discover sample collection devices. The purified DNA can be used directly in PCR, microarrays and next-generation sequencing applications.
The use of paramagnetic particles for DNA capture eliminates the need for centrifugation or vacuum manifolds, making the system suitable for full automation. In addition, the system does not require an organic solvent, making it safe and convenient. DNA yields of up to 12µg are expected from input blood volumes of 350µl, depending on the white blood cell count of the sample. Saliva samples can have variable amounts of gDNA, and up to 18µg or more of DNA may be recovered from a 700µl Oragene® collection device sample.
Features – Benefits
- Robust, precipitation-free protocol, No chance of “lost pellets”.
- High yields of pure DNA from pristine and challenged or hemolysed samples.
- Pure gDNA ready for demanding applications; samples in solution; no resuspension required
| Product | Size | Brand | Catalog |
| Maxwell® HT 96 gDNA Blood Isolation System | 1 x 96 preps | Promega | A2670 |
| Maxwell® HT 96 gDNA Blood Isolation System | 4 x 96 preps | Promega | A2671 |
Specifications
| Item | Size | Catalog |
| Binding Buffer (BBA) | 1 × 50ml | A174B |
| Resin | 1 × 5.5ml | A175B |
| Wash Buffer (WBA) | 1 × 85ml | A176B |
| 10mM EDTA (pH 8.0) | 1 × 10ml | A263A |
| Proteinase K (PK) Solution | 1 × 5.5ml | A505C |
| RNase A Solution 4 mg/ml | 1 × 1ml | A797C |
| 25mM Tris-HCl (pH 8.0) | 1 × 60 ml | A2641 |
| Cell Lysis Buffer (CLD) | 1 × 40ml | A173B |
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