Description
Emergence of new viral diseases, including the coronavirus SARS-CoV-2 (COVID 19), MERS (2015) and SARS (2003) outbreaks, highlight the need for fast methods to detect and identify target viruses at scale. The Maxwell® HT Viral TNA Kit can be used for high-throughput, automated extraction of RNA and DNA from several sample types, including universal transport medium, serum, plasma and others. Samples are processed in 96-well plates using magnetic separation. The Maxwell® HT purification kit can also be used in manual protocols using a multichannel pipettor. First, Lysis buffer will be combined with Proteinase K. After that, sample will be added to the combination. Second, DNA/RNA is captured on Resin PMPs, then will be released and washed. Lastly, total RNA and DNA will be recovered. Isolated total DNA and RNA can be used for qPCR, RT-qPCR, ddPCR, and next generation sequencing. From the box, you will find MagneSil® RED, 4/40 Wash Solution, Proteinase K Solution, alcohol wash, nuclease-free water, and lysis buffer.
Features and Benefits
- Robust protocol supports various viral sample types and a range of input volumes.
- Magnetic separation avoids the use of solvents.
- Extraction of RNA and DNA from viral samples such as universal transport medium, serum, plasma and others.
- Compatibility: with several liquid handler, example King’s Fisher, Teccan, Eppendorf, etc.
Product | Size | Brand | Catalog |
Maxwell® HT Viral TNA Kit, Custom | 4 × 96 preps | Promega | AX2340 |
Specifications
Item | Size | Catalog |
MagneSil® RED | 1 × 60ml | A164B |
4/40 Wash Solution | 1 × 115ml | A2221 |
Proteinase K (PK) Solution | 1 × 16ml | MC500E |
Alcohol Wash, Blood | 1 × 70ml | MD1411 |
Lysis Buffer | 1 × 160ml | MC5018 |
Nuclease-Free Water | 1 × 150ml | P1195 |
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